Nucleotide sequence of the 5'-flanking region of the murine ornithine decarboxylase gene.

We have sequenced a 1674basepair Pst I fragment ( 1 6 5 8 to +16, relative to the transcription start site) from the 5"-f1ank1ng region of the mouse ornithine decarboxylase (ODC) gene, Isolated from a genomtc library of BALB/c mice (generously provided by Dr. J. V. Ravetch). Previously, Brabant et al. ( 1 ) and Katz and Kahane ( 2 ) have reported sequences for the 5'-flank1ng region of this gene, up to positfon 3 6 4 and 5 3 6 , respectively. Our sequence, shown below, is identical with that reported by Brabant et el. ( 1 ) ; sequence dissimilarities with Ketzand Kahana (2 ) are denoted by solMI underlines. The sequence from -537 to 1 6 5 8 of the mouse ODC gene has not been reported previously. Several potential transcriptional control elements are present in this gene (dotted underlines), Including 3 A P 1 binding sites containing the characteristic TGAXTCA motif ( 3 ) , and 2 estrogen-response elements containing a single nt mismatch within the characteristic palindromtc sequence (4 ) . Comparison with the rat ODC 5'flanking region ( 5 ) revealed several areas of high sequence similarity, most notably a 91.8!8 identity in a 377 nt overlap In the sequence Immediately adjacent to the transcription start site In both genes.